Difference between revisions of "Part:BBa K565001"

 
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In our project, we used Colicin G to kill pathogen cells (in our case they were E. coli DH5alpha strain) by membrane lysis, desinfecting the contaminated water.
 
In our project, we used Colicin G to kill pathogen cells (in our case they were E. coli DH5alpha strain) by membrane lysis, desinfecting the contaminated water.
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'''Bibliography'''
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Cascales E. et al. Colicin Biology. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, Mar. 2007, p. 158–229 Vol. 71, No. 1.
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Patzer S. I. et al. The colicin G, H and X determinants encode microcins M and H47, which might utilize the catecholate siderophore receptors FepA, Cir, Fiu and IroN. Microbiology (2003), 149, 2557–2570.
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Bradley D. E. 1991. Colicins G and H and their host strains. Can. J. Microbiol. 37: 751-757.
  
  

Revision as of 16:19, 21 September 2011

Colicin G gene cluster

Colicin G is a protein produced by Escherichia coli CA46 strain. They make holes in bacterial membranes, being lethal for related strains of E. coli.

The Colicins G proteins have specific activity, they need the presence of Fiu protein in the outer membrane of target cells in order to recognize them, and use the TonB/ExbB, D translocation route to slip in the cell and lyse it.

In our project, we used Colicin G to kill pathogen cells (in our case they were E. coli DH5alpha strain) by membrane lysis, desinfecting the contaminated water.

Bibliography

Cascales E. et al. Colicin Biology. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, Mar. 2007, p. 158–229 Vol. 71, No. 1.

Patzer S. I. et al. The colicin G, H and X determinants encode microcins M and H47, which might utilize the catecholate siderophore receptors FepA, Cir, Fiu and IroN. Microbiology (2003), 149, 2557–2570.

Bradley D. E. 1991. Colicins G and H and their host strains. Can. J. Microbiol. 37: 751-757.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 906
    Illegal EcoRI site found at 3673
    Illegal EcoRI site found at 4423
    Illegal EcoRI site found at 4968
    Illegal EcoRI site found at 7426
    Illegal EcoRI site found at 9490
    Illegal XbaI site found at 6611
    Illegal PstI site found at 1611
    Illegal PstI site found at 5169
    Illegal PstI site found at 5178
    Illegal PstI site found at 5720
    Illegal PstI site found at 8418
    Illegal PstI site found at 8458
    Illegal PstI site found at 8631
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 906
    Illegal EcoRI site found at 3673
    Illegal EcoRI site found at 4423
    Illegal EcoRI site found at 4968
    Illegal EcoRI site found at 7426
    Illegal EcoRI site found at 9490
    Illegal PstI site found at 1611
    Illegal PstI site found at 5169
    Illegal PstI site found at 5178
    Illegal PstI site found at 5720
    Illegal PstI site found at 8418
    Illegal PstI site found at 8458
    Illegal PstI site found at 8631
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 906
    Illegal EcoRI site found at 3673
    Illegal EcoRI site found at 4423
    Illegal EcoRI site found at 4968
    Illegal EcoRI site found at 7426
    Illegal EcoRI site found at 9490
    Illegal BglII site found at 3519
    Illegal BglII site found at 6011
    Illegal BglII site found at 6174
    Illegal BamHI site found at 7419
    Illegal BamHI site found at 7472
    Illegal BamHI site found at 8045
    Illegal BamHI site found at 10157
    Illegal BamHI site found at 11618
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 906
    Illegal EcoRI site found at 3673
    Illegal EcoRI site found at 4423
    Illegal EcoRI site found at 4968
    Illegal EcoRI site found at 7426
    Illegal EcoRI site found at 9490
    Illegal XbaI site found at 6611
    Illegal PstI site found at 1611
    Illegal PstI site found at 5169
    Illegal PstI site found at 5178
    Illegal PstI site found at 5720
    Illegal PstI site found at 8418
    Illegal PstI site found at 8458
    Illegal PstI site found at 8631
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 906
    Illegal EcoRI site found at 3673
    Illegal EcoRI site found at 4423
    Illegal EcoRI site found at 4968
    Illegal EcoRI site found at 7426
    Illegal EcoRI site found at 9490
    Illegal XbaI site found at 6611
    Illegal PstI site found at 1611
    Illegal PstI site found at 5169
    Illegal PstI site found at 5178
    Illegal PstI site found at 5720
    Illegal PstI site found at 8418
    Illegal PstI site found at 8458
    Illegal PstI site found at 8631
    Illegal AgeI site found at 102
    Illegal AgeI site found at 1081
    Illegal AgeI site found at 10538
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 9124
    Illegal SapI site found at 10233
    Illegal SapI.rc site found at 6058
    Illegal SapI.rc site found at 6978