Difference between revisions of "Part:BBa K608151"
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We designed this composite in order to gain the enzyme ''pcyA'' (phycocyanobilin-ferredoxin oxidoreductase),<br/> | We designed this composite in order to gain the enzyme ''pcyA'' (phycocyanobilin-ferredoxin oxidoreductase),<br/> | ||
| − | this design is necessary if the part is going be integrated into an assembly of various genes.<br/> | + | this design is necessary if the part is going be integrated into an assembly of various genes.<br/>[[Image:Enz.jpg|thumb|300px]scheme of enzymes] |
The enzyme plays an important role in the production of the PCB chromophore (phycocyanobilin)<br/> | The enzyme plays an important role in the production of the PCB chromophore (phycocyanobilin)<br/> | ||
which is essential for the green and red light receptor system.<br/> | which is essential for the green and red light receptor system.<br/> | ||
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==Usage and Biology== | ==Usage and Biology== | ||
| − | + | ||
For PCB chromophore production the enzyme [https://parts.igem.org/Part:BBa_I15008 ho1] (heme oxygenase)<br/> in a similar design is necessary infront of this part<br/> | For PCB chromophore production the enzyme [https://parts.igem.org/Part:BBa_I15008 ho1] (heme oxygenase)<br/> in a similar design is necessary infront of this part<br/> | ||
because both enzymes are needed to convert heme into PCB chromophore.<br/> | because both enzymes are needed to convert heme into PCB chromophore.<br/> | ||
Revision as of 16:07, 21 September 2011
translational unit of pcyA
We designed this composite in order to gain the enzyme pcyA (phycocyanobilin-ferredoxin oxidoreductase),
this design is necessary if the part is going be integrated into an assembly of various genes.
[[Image:Enz.jpg|thumb|300px]scheme of enzymes]
The enzyme plays an important role in the production of the PCB chromophore (phycocyanobilin)
which is essential for the green and red light receptor system.
Our sequencing confirmed that the part is correct in the pSB1C3 vector.
All physical DNA samples used for this composite come from the registry distribution.
Usage and Biology
For PCB chromophore production the enzyme ho1 (heme oxygenase)
in a similar design is necessary infront of this part
because both enzymes are needed to convert heme into PCB chromophore.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 830
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 383
- 1000COMPATIBLE WITH RFC[1000]