Difference between revisions of "Part:BBa K584027"
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<p align="justify">This part contains the Constitutive Promoter (J23119) with the Ice Nucleating Protein Generator (BBa_K584024) behind it.</p>
 
<p align="justify">This part contains the Constitutive Promoter (J23119) with the Ice Nucleating Protein Generator (BBa_K584024) behind it.</p>
  
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<p align="justify">The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. '''inaZ''' is the gene responsible for producing the ice nucleation protein.</p>
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<p align="justify">The ice nucleating protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. '''inaZ''' is the gene responsible for producing the ice nucleation protein.</p>
  
 
<p align="justify">We would like to thank [http://www.academicbiography.uwo.ca/profile.php?&n=ggloor Prof. Dr. Gregory Gloor ], for providing us with a plasmid (pUC1813ICE) which contains the ice nucleating protein gene '''inaZ'''.</p>
 
<p align="justify">We would like to thank [http://www.academicbiography.uwo.ca/profile.php?&n=ggloor Prof. Dr. Gregory Gloor ], for providing us with a plasmid (pUC1813ICE) which contains the ice nucleating protein gene '''inaZ'''.</p>

Revision as of 15:49, 21 September 2011

Constitutive_Promoter + INP(Ice Nucleating Protein Generator)

This part contains the Constitutive Promoter (J23119) with the Ice Nucleating Protein Generator (BBa_K584024) behind it.

The ice nucleating protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. inaZ is the gene responsible for producing the ice nucleation protein.

We would like to thank [http://www.academicbiography.uwo.ca/profile.php?&n=ggloor Prof. Dr. Gregory Gloor ], for providing us with a plasmid (pUC1813ICE) which contains the ice nucleating protein gene inaZ.

To know more about the inaZ, it is suggested to read this [http://www.ncbi.nlm.nih.gov/pubmed/10933828 article] describing the usage of pUC1813ICE with the inaZ.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 707
    Illegal NgoMIV site found at 2219
    Illegal NgoMIV site found at 2363
    Illegal NgoMIV site found at 2627
    Illegal NgoMIV site found at 2771
    Illegal NgoMIV site found at 2819
    Illegal NgoMIV site found at 2939
    Illegal AgeI site found at 478
    Illegal AgeI site found at 1883
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 653
    Illegal BsaI.rc site found at 3399
    Illegal SapI.rc site found at 299