Difference between revisions of "Part:BBa C0083:Experience"

Revision as of 15:42, 21 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_C0083

User Reviews

UNIQ462d576edf6bd184-partinfo-00000000-QINU

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iGEM Dundee 2011

We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as BBa_K562017). This will allow better monitoring of production of the protein. We were able to detect production of this fusion in E. coli using an anti-HA monoclonal antibody. We did not get the chance to test fumarate and/or ammonia production in a host cell producing this part.

UNIQ462d576edf6bd184-partinfo-00000003-QINU

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-We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as <partinfo>K562018</partinfo>). This will allow better monitoring of production of the protein. Indeed, we could detect production of this fusion in <i>E. coli</i> using an anti-HA monoclonal antibody. We did not get the chance to test arsenic resistance in a host cell producing this part.
+We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as <partinfo>K562017</partinfo>). This will allow better monitoring of production of the protein. We were able to detect production of this fusion in <i>E. coli</i> using an anti-HA monoclonal antibody. We did not get the chance to test fumarate and/or ammonia production in a host cell producing this part.
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