Difference between revisions of "Part:BBa C0083:Experience"

 
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===User Reviews===
 
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<I>iGEM Groningen 2009</I>
 
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The capacity of fMT to enhance the arsenite accumulation in ''E. coli'' was tested using an arsenite uptake [http://2009.igem.org/Team:Groningen/Protocols assay]. But unfortunately the arsenic concentration determined by ICP-MS was not reproducible and did not show enhanced arsenite accumulation in ''E. coli'' carrying fMT (induced by lactose).
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<I>iGEM Dundee 2011</I>
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We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as <partinfo>K562018</partinfo>). This will allow better monitoring of production of the protein. Indeed, we could detect production of this fusion in <i>E. coli</i> using an anti-HA monoclonal antibody. We did not get the chance to test arsenic resistance in a host cell producing this part.
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Revision as of 15:39, 21 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_C0083

User Reviews

UNIQ69ccc899559fd5f4-partinfo-00000000-QINU

••

iGEM Groningen 2009

The capacity of fMT to enhance the arsenite accumulation in E. coli was tested using an arsenite uptake [http://2009.igem.org/Team:Groningen/Protocols assay]. But unfortunately the arsenic concentration determined by ICP-MS was not reproducible and did not show enhanced arsenite accumulation in E. coli carrying fMT (induced by lactose).

•••

iGEM Dundee 2011

We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as BBa_K562018). This will allow better monitoring of production of the protein. Indeed, we could detect production of this fusion in E. coli using an anti-HA monoclonal antibody. We did not get the chance to test arsenic resistance in a host cell producing this part.

UNIQ69ccc899559fd5f4-partinfo-00000004-QINU