Difference between revisions of "Part:BBa K592201"

 
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<partinfo>BBa_K592201 short</partinfo>
 
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BBa_K592201 is a BioBrick friendly vector with low to medium copy p15A replication origin (BBa_I50032) and chloramphenicol antibiotic resistance marker, usable for Lambda Red recombineering in E coli. The E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22, and the chloramphenicol resistance marker is flanked by FRT sites so that the resistance marker can be removed after chromosomal integration using the FLP recombinase.
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BBa_K592201 is a BioBrick friendly vector with low to medium copy p15A replication origin (BBa_I50032) and chloramphenicol antibiotic resistance marker, usable for Lambda Red recombineering in ''E coli''. The ''E coli'' His operon terminator BBa_B0053 has been replaced with the late terminator of the ''Salmonella'' phage P22, and the chloramphenicol resistance marker is flanked by FRT sites so that the resistance marker can be removed after chromosomal integration using the FLP recombinase.
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[[Image:K592201-J04450.png]]
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Revision as of 15:37, 21 September 2011

Low to medium copy Lambda Red recombineering compatible plasmid

BBa_K592201 is a BioBrick friendly vector with low to medium copy p15A replication origin (BBa_I50032) and chloramphenicol antibiotic resistance marker, usable for Lambda Red recombineering in E coli. The E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22, and the chloramphenicol resistance marker is flanked by FRT sites so that the resistance marker can be removed after chromosomal integration using the FLP recombinase.


K592201-J04450.png


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1806
    Illegal XbaI site found at 2736
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2946
    Illegal NheI site found at 1403
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2952
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2946
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2946
    Illegal suffix found at 2
    Illegal XbaI site found at 1806
    Illegal XbaI site found at 2736
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2946
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1806
    Illegal XbaI site found at 2736
    Illegal XbaI site found at 2961
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 994
    Illegal AgeI site found at 1317
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 289