Difference between revisions of "Part:BBa K608352"

Revision as of 15:36, 21 September 2011

Bacteriophage Lysis Cassette with RBS

Bacteriophage Lysis cassette based on the part (BBa_K124017) (note the overlapping coding regions of the sequence in spite of the given sequence for (BBa_K124017)) with an additional strong RBS (BBa_B0034)

Why did we make this part?: Because BBa_K124017 had no RBS (was only a CDS) and also because of the nonexistent annotation of that sequence, as well as the fact that it showed no overlapping regions, which could lead to confusion in the future.

Usage and Biology

The phage lysis cassette consists of the standard bacteriophage λ lysis genes S/S’, R and Rz/Rz1, coded in overlapping sequences with phase 1 and 2 frameshifts.

• S: λ Anti-Holin
• S’: λ Holin
• R: λ Endolysin
• Rz: Putative type-II signal anchor protein
• Rz1: Outer membrane lipoprotein

The phage λ lysozyme (R Endolysin) hydrolyses the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc), but in order to degrade the cell wall it requires a small transmembrane protein called holin which perforates the membrane for endolysin to gain access to the murein [Ry Young et al]. The auxiliary lysis proteins Rz and Rz1 have long been known to play a vital role but have yet to be assigned a specific function stemming from functional and structural analysis. The assumption is that they form a complex spanning the periplasm and fuse the outer and inner membranes, removing the last physical barrier for cell lysis [Joel Berry et al].

References

Ry Young et al
“Phages will out: strategies of host cell lysis”
Trends in Microbiology Vol 8, Issue 3 (2000) 120-128

Joel Berry et al
“The final step in the phage infection cycle: the Rz and Rz1 lysis proteins link the inner and outer membranes”
Molecular Microbiology 70 (2008) 341–351

Sequence and Features