Difference between revisions of "Part:BBa K549003"

 
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<partinfo>BBa_K549003 short</partinfo>
 
<partinfo>BBa_K549003 short</partinfo>
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The biobrick contains the RcnA promotor from E. coli fused with the luxAB reporter gene. E coli strains own the RcnR-regulator gene which direct regulates the transcription of the RcnA nickel cobalt efflux protein from which the promotor is amplified. In presence of nickel the Luciferase is expressed.
 
The biobrick contains the RcnA promotor from E. coli fused with the luxAB reporter gene. E coli strains own the RcnR-regulator gene which direct regulates the transcription of the RcnA nickel cobalt efflux protein from which the promotor is amplified. In presence of nickel the Luciferase is expressed.
  
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<!-- Add more about the biology of this part here
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The promoter is also a little bit sensitive for Co 2+.
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This is how the BioBrick is working:
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[[Image:Bild-rcnaluxAB]]
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===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 15:35, 21 September 2011

Nickel(II) dependent promotor with luxAB as reporter

The biobrick contains the RcnA promotor from E. coli fused with the luxAB reporter gene. E coli strains own the RcnR-regulator gene which direct regulates the transcription of the RcnA nickel cobalt efflux protein from which the promotor is amplified. In presence of nickel the Luciferase is expressed.

The promoter is also a little bit sensitive for Co 2+.

This is how the BioBrick is working: File:Bild-rcnaluxAB


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 751
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1270