Difference between revisions of "Part:BBa I742111:Experience"

Revision as of 15:34, 21 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I742111

A composite LIMS1 part with a Lac promoter has been characterized. For details, please visit this page.

User Reviews

UNIQ786c0cbe34f53aeb-partinfo-00000001-QINU

•••

iGEM Dundee 2011

We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as BBa_K562015). This will allow better monitoring of production of the protein, and it was detectable using an anti-HA monoclonal antibody whenexpressed in E. coli. We did not get the chance to test limonene production in a host cell producing this part.

UNIQ786c0cbe34f53aeb-partinfo-00000004-QINU

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-We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as <partinfo>K562015</partinfo>). This will allow better monitoring of production of the protein, and it was detectable using an anti-HA monoclonal antibody. We did not get the chance to test limonene production in a host cell producing this part.
+We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as <partinfo>K562015</partinfo>). This will allow better monitoring of production of the protein, and it was detectable using an anti-HA monoclonal antibody whenexpressed in <i>E. coli</i>. We did not get the chance to test limonene production in a host cell producing this part.
 |}
 ;
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 <!-- DON'T DELETE --><partinfo>BBa_K190019 EndReviews</partinfo>