Difference between revisions of "Part:BBa K549001"

(Usage and Biology)
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This BioBrick can be used to detect Ni(II)-Ions in water.  
 
This BioBrick can be used to detect Ni(II)-Ions in water.  
  
 +
This is how it works:
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 +
[[File:Bild-rcnA-GFP]]
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 +
The detector is also sensing Co 2+, but not very sensitivly.
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Sources:
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- [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2006.05369.x/pdf Nickel homeostasis in Escherichia coli – the rcnR-rcnA efflux pathway and its linkage to NikR function] Iwig JS, Rowe JL, Chivers PT <br>
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- [http://www.ncbi.nlm.nih.gov/pubmed?term=coordinating%20intracellular%20chivers Coordinating intracellular nickel-metal-site structure-function relationships and the NikR and RcnR repressors] Iwig JS, Chivers PT<br>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:26, 21 September 2011

Ni(II) dependent rcnA-promoter and GFP

E. coli has an RcnR-regulator that switches on the RcnA-Promotor in the presence of Ni(II). After induction GFP will be expressed.

E. coli has to be used as reporter organism since RcnR is not coded on the plasmid.

Usage and Biology

This BioBrick can be used to detect Ni(II)-Ions in water.

This is how it works:

File:Bild-rcnA-GFP

The detector is also sensing Co 2+, but not very sensitivly.

Sources: - [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2006.05369.x/pdf Nickel homeostasis in Escherichia coli – the rcnR-rcnA efflux pathway and its linkage to NikR function] Iwig JS, Rowe JL, Chivers PT
- [http://www.ncbi.nlm.nih.gov/pubmed?term=coordinating%20intracellular%20chivers Coordinating intracellular nickel-metal-site structure-function relationships and the NikR and RcnR repressors] Iwig JS, Chivers PT

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 863