Difference between revisions of "Part:BBa K608351"

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heat sensitive cI QPI with high promoter, with 6 instead of 8bp between the RBS and cI repressor ATG
 
heat sensitive cI QPI with high promoter, with 6 instead of 8bp between the RBS and cI repressor ATG
  
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
  
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===Usage and Biology===
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{| style="color:black; width="75%" cellpadding="10%" cellpadding="15%" cellspacing="0" border="1" align=center
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|-
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| style="width: 50%;background-color:lightgrey;" |
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; [[Image:Freiburg11 Presentation1.jpg|350px]]
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The temperature sensitive promoter functioning at 28-30°C
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| style="width: 50%;background-color:lightgrey;" |
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;
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[[Image:Freiburg11 Presentation3.jpg|350px]]
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The temperature sensitive promoter functioning at 42°C
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|}
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<br>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 15:14, 21 September 2011

(correct) temperature sensitive promoter

heat sensitive cI QPI with high promoter, with 6 instead of 8bp between the RBS and cI repressor ATG


Usage and Biology

Freiburg11 Presentation1.jpg

The temperature sensitive promoter functioning at 28-30°C

Freiburg11 Presentation3.jpg The temperature sensitive promoter functioning at 42°C


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 781
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]