Difference between revisions of "Part:BBa I746907:Experience"
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====results==== | ====results==== | ||
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| + | part works well | ||
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| + | induction t(1/2) = ca. 200 min | ||
no concentration dependency of GFP induction at 1.5 to 0.1 mM IPTG, as IPTG concentration was in saturation range | no concentration dependency of GFP induction at 1.5 to 0.1 mM IPTG, as IPTG concentration was in saturation range | ||
Revision as of 15:04, 21 September 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_I746907
User Reviews
TU_Munich Team 2011
We tested the part and it worked very well.
TU_Munich Team 2011 GFP Assay
I746907 (T7 promotor with GFP) in pSB1A2 in BL21DE3 cells
change of medium to M63 (LB shows slight fluorescence itself)
five concentrations of IPTG in the medium were tested (1.5, 1.0, 0.5, 0.25, 0.1 mM IPTG) growth of cells at 37 °C, shaking
excitation filter: 485 nm, emission filter: 520 nm
Problem: measurement of Absorbance only every 100 minutes led to spikes in the diagram (Fluorescence measured every 10 min.)
results
part works well
induction t(1/2) = ca. 200 min
no concentration dependency of GFP induction at 1.5 to 0.1 mM IPTG, as IPTG concentration was in saturation range
for this diagram:
- calculation: Fluorescence/Absorbance
- mean of triplicate measurement
- adjusted to 100 %
- corrected for outliers
- shortened to 700 min
UNIQ9e2a372b8375454f-partinfo-00000000-QINU
UNIQ9e2a372b8375454f-partinfo-00000001-QINU