Difference between revisions of "Part:BBa K608352"
(→Usage and Biology) |
|||
| Line 6: | Line 6: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | It consists of the standard bacteriophage λ lysis genes, ie S/S’, R, Rz/Rz1, coded in overlapping sequences with phase 1 and 2 frameshifts. | ||
| + | {| style="color:black; background-color:lightgrey;" cellpadding="10%" cellpadding="15%" cellspacing="0" border="1" align="right" | ||
| + | |[[File:Freigem11 Präsentation3.jpg|caption|200px]] | ||
| + | Bacteriophage λ lysis genes at work | ||
| + | |} | ||
| + | <br> | ||
| + | *S: λ Anti-Holin | ||
| + | *S’: λ Holin | ||
| + | *R: λ Endolysin | ||
| + | *Rz: Putative type-II signal anchor protein | ||
| + | *Rz1: Outer membrane lipoprotein | ||
| + | |||
| + | <br> | ||
| + | |||
| + | The phage λ lysozyme (R Endolysin) hydrolyses the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc), but in order to degrade the cell wall it requires a small transmembrane protein called holin which perforates the membrane for endolysin to gain access to the murein [Ry Young et al]. The auxiliary lysis proteins Rz and Rz1 have long been known to play a vital role but have yet to be assigned a specific function stemming from functional and structural analysis. The assumption is that they form a complex spanning the periplasm and fuse the outer and inner membranes, removing the last physical barrier for cell lysis [Joel Berry et al]. | ||
[[Image:Freigem11 Presentation3.jpg|500px]] | [[Image:Freigem11 Presentation3.jpg|500px]] | ||
<br> | <br> | ||
Revision as of 14:44, 21 September 2011
Bacteriophage Lysis Cassette with RBS
Bacteriophage Lysis cassette based on the part BBa_K124017 (note the overlapping coding regions of the sequence in spite of the given sequence for BBa_K124017) with an additional strong RBS (BBa_B0034)
Usage and Biology
It consists of the standard bacteriophage λ lysis genes, ie S/S’, R, Rz/Rz1, coded in overlapping sequences with phase 1 and 2 frameshifts.
Bacteriophage λ lysis genes at work |
- S: λ Anti-Holin
- S’: λ Holin
- R: λ Endolysin
- Rz: Putative type-II signal anchor protein
- Rz1: Outer membrane lipoprotein
The phage λ lysozyme (R Endolysin) hydrolyses the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc), but in order to degrade the cell wall it requires a small transmembrane protein called holin which perforates the membrane for endolysin to gain access to the murein [Ry Young et al]. The auxiliary lysis proteins Rz and Rz1 have long been known to play a vital role but have yet to be assigned a specific function stemming from functional and structural analysis. The assumption is that they form a complex spanning the periplasm and fuse the outer and inner membranes, removing the last physical barrier for cell lysis [Joel Berry et al].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]