Difference between revisions of "Part:BBa K627011:Design"
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===Source=== | ===Source=== | ||
− | + | TorA signal sequence and the lactamase was amplified via PCR from pJC354, which originates from two iGEM compatible BioBricks: BBa_K208005 and BBa_I757010. The cleavage site was created, based on the information of the Brenda enzymes database, via 2 oligonucleotides ordered from Sigma-Aldrich. | |
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===References=== | ===References=== |
Revision as of 14:40, 21 September 2011
Fusion part of pBAD arabinose-inducible induction system and the HRV 14_3C protease
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1523
Illegal BglII site found at 1711
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1239
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
This biobrick was built by PCR using the following PCR primers:
Forward primer (p_TorA-f): CTTCTAGATGAACAATAACGATCTCTTTCAGGCATC
Reverse primer (o_bla_igem_r): CTACTAGTATTAACCGGTCCAATGCTTAATCAGTGAGGCAC
Source
TorA signal sequence and the lactamase was amplified via PCR from pJC354, which originates from two iGEM compatible BioBricks: BBa_K208005 and BBa_I757010. The cleavage site was created, based on the information of the Brenda enzymes database, via 2 oligonucleotides ordered from Sigma-Aldrich.