Difference between revisions of "Part:BBa I746907:Experience"
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[[Image:2011-09-19 GFP Assay Diagramm prozentual.jpg]] | [[Image:2011-09-19 GFP Assay Diagramm prozentual.jpg]] | ||
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I746907 (T7 promotor with GFP) in pSB1A2 in BL21DE3 cells | I746907 (T7 promotor with GFP) in pSB1A2 in BL21DE3 cells | ||
Revision as of 14:25, 21 September 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_I746907
User Reviews
TU_Munich Team 2011
We tested the part and it worked very well.
our GFP Assay
I746907 (T7 promotor with GFP) in pSB1A2 in BL21DE3 cells
change of medium to M63 (LB shows slight fluorescence itself)
Problem: measurement of Absorbance only every 100 minutes led to spikes in the diagram
results
no concentration dependency of GFP induction as IPTG concentration was in saturation range
for this diagram:
- calculated: Fluorescence/Absorbance
- mean of triplicate measurement
- adjusted to 100 %
- corrected for outliers
- shortened to 700 min
UNIQ0e2eac7cc310feb0-partinfo-00000000-QINU
UNIQ0e2eac7cc310feb0-partinfo-00000001-QINU