Difference between revisions of "Part:BBa K551000"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K551000 short</partinfo>
 
<partinfo>BBa_K551000 short</partinfo>
  
−
This part is composed of two Flipase Recognition Targets (FRT) flanking the Chloramphenicol resistance gene.
+
This part is composed of two mutated Flipase Recognition Targets (FRT') flanking the Chloramphenicol resistance gene. The mutation removes the EcoRI restriction site from the canonical FRT sequence. It is the same as BBa_J61025 but without the scar between the first FRT' and CmR.
 +
 
 +
The two FRT' sequences allow the excision of the flanked sequence by the Flipase recombinase.
 +
 
 +
It's been tested with success for resistance to Chloramphenicol and excision per the pINDEL plasmid.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
It can be used to give Chloramphenicol resistance and/or as a removable selection marker with the help of pINDEL or pCP20.
 +
  
 
<!-- -->
 
<!-- -->

Revision as of 13:08, 21 September 2011

FRT-CM-FRT

This part is composed of two mutated Flipase Recognition Targets (FRT') flanking the Chloramphenicol resistance gene. The mutation removes the EcoRI restriction site from the canonical FRT sequence. It is the same as BBa_J61025 but without the scar between the first FRT' and CmR.

The two FRT' sequences allow the excision of the flanked sequence by the Flipase recombinase.

It's been tested with success for resistance to Chloramphenicol and excision per the pINDEL plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]