Difference between revisions of "Part:BBa K551000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The initial part wasn't working, so we remade it from the intermediate | + | The initial part (BBa_J61025) wasn't working, so we remade it from the intermediate CmR-FRT (BBa_J61024). For that we made a PCR with a primers containing the FRT sequence and the RFC10 prefix; this means there is no scar between the first FRT and CmR. |
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===Source=== | ===Source=== | ||
Revision as of 12:57, 21 September 2011
FRT-CM-FRT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The initial part (BBa_J61025) wasn't working, so we remade it from the intermediate CmR-FRT (BBa_J61024). For that we made a PCR with a primers containing the FRT sequence and the RFC10 prefix; this means there is no scar between the first FRT and CmR.
Source
It comes from PCR of a registry part