Difference between revisions of "Part:BBa K660200"
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K660200 short</partinfo>
 
<partinfo>BBa_K660200 short</partinfo>
−
<h2>Origin</h2>
+
<h3>Origin</h3>
 
Horse sweat <i>(Equus caballus)</i><br/>
 
Horse sweat <i>(Equus caballus)</i><br/>
  
−
<h2>Function</h2>
+
<h3>Function</h3>
−
Latherin is a surfactant protein. Its normal biological function is temperature regulation and is believed to function by enhancing evaporation from the pelt. Due to its ability to bind to hydrophobic surfaces, the protein is being investigated for its possible ability to aid in breaking up biofilms.
+
Latherin is a surfactant protein with detergent like properties. <br/>
  
−
<h2>Additional Information</h2>
+
Its normal biological function is temperature regulation and is believed to function by enhancing evaporation from the pelt. It does so by binding to hydrophobic surfaces and allowing them to become wettable.  <Br/>
 +
 
 +
<h3>Applications</h3>
 +
Latherin is able to temporarily bind to hydrophobic surfaces and has therefore been investigated as a possible method of breaking up biofilms. It is also suspected that the protein may have antimicrobial functions.  <br/>
 +
The 2011 University of Glasgow iGEM team used this part in their system, DISColi, to aid in the dispersal of biofilms.
 +
 
 +
<h3>Additional Information</h3>
 
Note: Latherin is suspected to be the cause of allergic reaction in individuals with an allergy to horses.<br/>
 
Note: Latherin is suspected to be the cause of allergic reaction in individuals with an allergy to horses.<br/>
  
−
<h2>Illegal Restriction Sites</h2>
+
<h3>Illegal Restriction Sites</h3>
  
 
We are aware of two illegal restriction sites that exist in our latherin biobrick.  Due to time constraints, we were unable to complete site directed mutagenesis. The primers we designed for this are below, for any future team.<br/><br/>
 
We are aware of two illegal restriction sites that exist in our latherin biobrick.  Due to time constraints, we were unable to complete site directed mutagenesis. The primers we designed for this are below, for any future team.<br/><br/>
  
−
1st PstI site:<br/>
+
<u>1st PstI site:</u><br/>
−
5': GCG GAA ATT AAA CTG CAA GAT ACC CGT CTG CAA CTG<br/>
+
5': GCG GAA ATT AAA CTG CAA GAT ACC CGT CTG CAA CTG<br/><br/>
  
−
  Forward Primer: 5' CAG TTG CAG ACG GGT ATC TTG CAG TTT AAT TTC CGC<br/>
+
&#160;&#160;&#160; Forward Primer: 5' CAG TTG CAG ACG GGT ATC TTG CAG TTT AAT TTC CGC<br/><br/>
 
     
 
     
−
  Reverse Primer: 5' GCG GAA ATT AAA CTG CAA GAT ACC CGT CTG CAA CTG<br/>
+
&#160;&#160;&#160; Reverse Primer: 5' GCG GAA ATT AAA CTG CAA GAT ACC CGT CTG CAA CTG<br/><br/>
 
   
 
   
 
MTP= 79.33  <br/><br/>
 
MTP= 79.33  <br/><br/>
  
  
−
2nd PstI site:<br/>
+
<u>2nd PstI site:</u><br/>
−
5': GCG ATT GAG CTG CAA AGC GGC AAT CCG C<br/>
+
5': GCG ATT GAG CTG CAA AGC GGC AAT CCG C<br/><br/>
  
−
Forward Primer: 5' G CGG ATT GCC GCT TTG CAG CTC AAT CGC<br/>
+
&#160;&#160;&#160; Forward Primer: 5' G CGG ATT GCC GCT TTG CAG CTC AAT CGC<br/><br/>
  
−
Reverse Primer: 5' GCG ATT GAG CTG CAA AGC GGC AAT CCG C<br/>
+
&#160;&#160;&#160; Reverse Primer: 5' GCG ATT GAG CTG CAA AGC GGC AAT CCG C<br/><br/>
  
−
MTP= 78.71</br></br>
+
MTP= 78.71<br/><br/>
  
 +
<h3>References & Further Reading</h3>
 +
Beegley J, et al., 1986. Isolation and characterization of latherin, a surface-active protein from horse sweat<br/>http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=search&term=3753435>.
 +
<br/><br/>
 +
Kennedy M., 2011., Latherin and other biocompatible surfactant proteins<br/>
 +
http://www.biochemsoctrans.org/bst/039/bst0391017.htm
  
  

Revision as of 12:56, 21 September 2011

Latherin (Surfactant Protein)

Origin

Horse sweat (Equus caballus)

Function

Latherin is a surfactant protein with detergent like properties.

Its normal biological function is temperature regulation and is believed to function by enhancing evaporation from the pelt. It does so by binding to hydrophobic surfaces and allowing them to become wettable.

Applications

Latherin is able to temporarily bind to hydrophobic surfaces and has therefore been investigated as a possible method of breaking up biofilms. It is also suspected that the protein may have antimicrobial functions.
The 2011 University of Glasgow iGEM team used this part in their system, DISColi, to aid in the dispersal of biofilms.

Additional Information

Note: Latherin is suspected to be the cause of allergic reaction in individuals with an allergy to horses.

Illegal Restriction Sites

We are aware of two illegal restriction sites that exist in our latherin biobrick. Due to time constraints, we were unable to complete site directed mutagenesis. The primers we designed for this are below, for any future team.

1st PstI site:
5': GCG GAA ATT AAA CTG CAA GAT ACC CGT CTG CAA CTG

    Forward Primer: 5' CAG TTG CAG ACG GGT ATC TTG CAG TTT AAT TTC CGC

    Reverse Primer: 5' GCG GAA ATT AAA CTG CAA GAT ACC CGT CTG CAA CTG

MTP= 79.33


2nd PstI site:
5': GCG ATT GAG CTG CAA AGC GGC AAT CCG C

    Forward Primer: 5' G CGG ATT GCC GCT TTG CAG CTC AAT CGC

    Reverse Primer: 5' GCG ATT GAG CTG CAA AGC GGC AAT CCG C

MTP= 78.71

References & Further Reading

Beegley J, et al., 1986. Isolation and characterization of latherin, a surface-active protein from horse sweat
http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=search&term=3753435>.

Kennedy M., 2011., Latherin and other biocompatible surfactant proteins
http://www.biochemsoctrans.org/bst/039/bst0391017.htm


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 200
    Illegal PstI site found at 431
    Illegal PstI site found at 569
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 200
    Illegal PstI site found at 431
    Illegal PstI site found at 569
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 514
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 200
    Illegal PstI site found at 431
    Illegal PstI site found at 569
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 200
    Illegal PstI site found at 431
    Illegal PstI site found at 569
  • 1000
    COMPATIBLE WITH RFC[1000]