Difference between revisions of "Part:BBa K568003"
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[[Image:TUM_2011_Miller_Assay_15.9.2011_of_BBa_K568003.jpg|left|thumb|400px|Results of the Miller Assay of BBa_K568003. The part was transcribed by genome-coded T7 polymerase, which was induced by addition of varying concentrations of IPTG. The control strain was not able to produce T7 polymerase.]]
 
[[Image:TUM_2011_Miller_Assay_15.9.2011_of_BBa_K568003.jpg|left|thumb|400px|Results of the Miller Assay of BBa_K568003. The part was transcribed by genome-coded T7 polymerase, which was induced by addition of varying concentrations of IPTG. The control strain was not able to produce T7 polymerase.]]
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Revision as of 12:52, 21 September 2011

T7 promoter lacZ reporter part

beta-galactosidase expression upon T7 polymerase binding


Usage and Biology

This part can be used as a reporter plasmid. It expresses beta-galactosidase if there is a T7 polymerase (or a mutant variant) nearby or transcribed through another part. The polymerase binds to the T7 promoter and transcription of the lacZ-Gene can occur, leading to beta-galactosidase expression.


Experimental Testing

The part was tested by the 2011 team of the TU_Munich on the 25th of September 2011. For this purpose, a Miller Assay was conducted. The results can be seen below.

Results of the Miller Assay of BBa_K568003. The part was transcribed by genome-coded T7 polymerase, which was induced by addition of varying concentrations of IPTG. The control strain was not able to produce T7 polymerase.


















Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]