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| − | ===Applications of BBa_K638402===
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| − | This is an improved version of [https://parts.igem.org/Part:BBa_K233307 part BBa_K233307] designed to allow comparison with measurements of functionality in the literature, and to make it easier to synthesise.
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| − | ===Producing BBa_k638402 from primer synthesis===
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| − | We assembled the basic part by using a pair of primers that will anneal to give the whole sequence. Biobrick prefixes and suffixes can then be added to create an in-frame fusion by assembly techniques such as [https://parts.igem.org/Plasmid_backbones/Assembly_of_protein_fusions BBF RFC 23 or 25]. Alternatively, Cambridge 2011 created scar-free fusions of this export tag to our protein of interest by [http://www.cambridgeigem.org/RFC57.pdf Gibson Assembly].
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| − | {| border="1" align="center" style="text-align:center;"
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| − | !Name
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| − | !Sequence
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| − | !Tm
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| − | |-
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| − | |TorA FWD
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| − | |ATGGCGAACAACGACTTATTTCAGGCTTCTCGGCGTCGCTTTCTGGCGCAGCTGGGCGGATTAACGGTGGCGGGT
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| − | |70.98°C
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| − | |-
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| − | |TorA REV
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| − | |TGCGGCTTGTGCTGCCGTCGCTCTGCGAGGAGTCAACAGCGACGGGCCCAACATACCCGCCACCGTTAATCCGCC
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| − | |70.98°C
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| − | |}
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| − | Thermocycler profile: 10 cycles:
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| − | Melt 95°C 10 sec /
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| − | Anneal 65°C 30 sec /
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| − | Extend 72°C 20 sec
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| | ===User Reviews=== | | ===User Reviews=== |
Latest revision as of 11:10, 21 September 2011
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UNIQ759b8cb41fa48147-partinfo-00000000-QINU
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