Difference between revisions of "Part:BBa K638402"
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<partinfo>BBa_K638402 short</partinfo> | <partinfo>BBa_K638402 short</partinfo> | ||
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| + | This is an improved version of [https://parts.igem.org/Part:BBa_K233307 part BBa_K233307] designed to allow comparison with measurements of functionality in the literature, and to make it easier to synthesise. | ||
This TorA leader sequence variant has had been successfully used to export GFP to the periplasm of E.coli as described [http://www.ncbi.nlm.nih.gov/pubmed/11123687 here]. | This TorA leader sequence variant has had been successfully used to export GFP to the periplasm of E.coli as described [http://www.ncbi.nlm.nih.gov/pubmed/11123687 here]. | ||
We ordered this sequence as a synthetic oligonucleotide in two halves: one 5-->3, one 3-->5, with 20bp overlap. We then joined these parts using PCR to make the full sequence. This is essentially a deliberate primer dimer. We inserted this sequence into our previous constructs using [http://www.synbio.org.uk/gibson/downloads/files/RFC57.pdf Gibson assembly], an extremely powerful method of assembling DNA constructs that is compatible with Standard Assembly. | We ordered this sequence as a synthetic oligonucleotide in two halves: one 5-->3, one 3-->5, with 20bp overlap. We then joined these parts using PCR to make the full sequence. This is essentially a deliberate primer dimer. We inserted this sequence into our previous constructs using [http://www.synbio.org.uk/gibson/downloads/files/RFC57.pdf Gibson assembly], an extremely powerful method of assembling DNA constructs that is compatible with Standard Assembly. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 11:04, 21 September 2011
TorA tag variant
This is an improved version of part BBa_K233307 designed to allow comparison with measurements of functionality in the literature, and to make it easier to synthesise.
This TorA leader sequence variant has had been successfully used to export GFP to the periplasm of E.coli as described [http://www.ncbi.nlm.nih.gov/pubmed/11123687 here].
We ordered this sequence as a synthetic oligonucleotide in two halves: one 5-->3, one 3-->5, with 20bp overlap. We then joined these parts using PCR to make the full sequence. This is essentially a deliberate primer dimer. We inserted this sequence into our previous constructs using [http://www.synbio.org.uk/gibson/downloads/files/RFC57.pdf Gibson assembly], an extremely powerful method of assembling DNA constructs that is compatible with Standard Assembly.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]