Difference between revisions of "Part:BBa K613001"
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See also parts K613007 through K613012 for the same T7 promoter variants with a lac operator to add LacI repression. | See also parts K613007 through K613012 for the same T7 promoter variants with a lac operator to add LacI repression. | ||
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Revision as of 10:04, 21 September 2011
T7 promoter family member
Parts K613001 through K613006 are T7 promoter variants. K613001 is the T7 promoter wildtype. The other sequences were derived by single base pair mutations that have been shown to alter its strength. The part includes a strong ribosomal binding site. Its strength was determined by cloning it in front of mRFP1 reporter and measuring fluorescence of BL21 cells containing a low copy number plasmid containing the reporter construct.
See also parts K613007 through K613012 for the same T7 promoter variants with a lac operator to add LacI repression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 49