Difference between revisions of "Part:BBa K342003"
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| + | <br><p><b>Conclusion</b>: The OD600 seemed to be the same for the two strains. There is no effect of the plasmid on the growth.</p> | ||
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Revision as of 09:13, 21 September 2011
OmpR234 protein, with higher effect on Curli promoter
This part is coding for a mutated protein OmpR. This protein will be phosphorylated by the complex EnvZ. This mutated phosphorylated protein is a better activator of the Curli promoter than OmpR.
Usage and Biology
This part can be used to induce a constitutive biofilm producing phenotype in bacteria.
Characterization
For characterization, the plasmids are introduced in PHL1414 strain. The strain with pIG16 plasmid (18A-OmpR234 in pSB1A2) is noted S31 and the control strain with the pIG3 plasmid (18A part in pSB1A2).
The effect of the plasmid on the adherence is measured.
To that end the strains are seeded in 24 wells plate.
The measure of the OD 600 will give the percentage of adherence of the bacteria and the violet crystal coloration will give us a first idea on the formation of biofilm.
Conclusion: The OD600 seemed to be the same for the two strains. There is no effect of the plasmid on the growth.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 137
- 1000COMPATIBLE WITH RFC[1000]