Difference between revisions of "Part:BBa K342003"
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This part is coding for a mutated protein OmpR. This protein will be phosphorylated by the complex EnvZ. This mutated phosphorylated protein is a better activator of the Curli promoter than OmpR. | This part is coding for a mutated protein OmpR. This protein will be phosphorylated by the complex EnvZ. This mutated phosphorylated protein is a better activator of the Curli promoter than OmpR. | ||
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=== Characterization === | === Characterization === | ||
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<img src="http://i.imgur.com/lF1O2.png" width="600px"> | <img src="http://i.imgur.com/lF1O2.png" width="600px"> | ||
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Revision as of 08:54, 21 September 2011
OmpR234 protein, with higher effect on Curli promoter This part is coding for a mutated protein OmpR. This protein will be phosphorylated by the complex EnvZ. This mutated phosphorylated protein is a better activator of the Curli promoter than OmpR. ===Usage and Biology===
This part can be used to induce a constitutive biofilm producing phenotype in bacteria.
=== Characterization ===
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 137
- 1000COMPATIBLE WITH RFC[1000]