Difference between revisions of "Part:BBa K364320:Experience"
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[[Image:Team Debrecen WB pCDNA-Gal4-NHR8.jpg|800px|thumb|center|Picture of Western blot: Gal4-NHR8 composite construct is highly expressed after transferring it into pCDNA3.1]]
 
[[Image:Team Debrecen WB pCDNA-Gal4-NHR8.jpg|800px|thumb|center|Picture of Western blot: Gal4-NHR8 composite construct is highly expressed after transferring it into pCDNA3.1]]
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[[Image:nhr64.jpg|center|600px|thumb|Activation of Gal-NHR8 and Gal-NHR64 to oil sand extractions.]]
  
 
===User Reviews===
 
===User Reviews===

Revision as of 07:42, 21 September 2011


Physiological effects of NHR-8

NHR-8 is required for gut function and may respond to the food signal.

The nhr-8 gene mutants have abnormally low resistance to the toxins colchicine and chloroquine.This resembles the regulation of protective metabolic enzymes by xenobiotic-sensing nuclear receptors.

NHR-8 may thus mediate a subset of the resistance traits promoted by PGP-3.

Possible applications of GAL4-NHR-8

By the addition of lipid ligands(unknown), the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).

Applications of BBa_K364320

Picture of gel electrophoresis: Gal4-NHR8 in pCDNA3.1 resulting an insert of 953 bp.
Picture of Western blot: Gal4-NHR8 composite construct is highly expressed after transferring it into pCDNA3.1
Activation of Gal-NHR8 and Gal-NHR64 to oil sand extractions.

User Reviews

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