Difference between revisions of "Part:BBa K539711:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding region, those RE site should not exist in the coding region.There are two Pst1 site, one Xba1 site and one EcoR1 site in ilvC. Those RE site in the part has been modified by point mutation.
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Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding region, those RE site should not exist in the coding region.There are two EcoR1 site in original sequence of ilvC. Those RE site in the ilvC has been modified by point mutation.
  
 
===Source===
 
===Source===

Revision as of 03:42, 21 September 2011

ilvC (ketol-acid reductoisomerase NAD(P)-binding)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 592
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 178
    Illegal BsaI.rc site found at 997


Design Notes

Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding region, those RE site should not exist in the coding region.There are two EcoR1 site in original sequence of ilvC. Those RE site in the ilvC has been modified by point mutation.

Source

It comes from Escherichia coli str. K-12 substr. MG1655


References