Difference between revisions of "Part:BBa K539714:Design"

 
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===Design Notes===
 
===Design Notes===
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In order to design the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding region.However, there is one RE site, EcoR1, in kivd. The RE site in kivd has been modified by point mutation.
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Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding sequence, those RE site should not exist in the coding region.There is one EcoR1 site in kivd. The RE site in the part has been modified by point mutation.
  
  

Revision as of 03:16, 21 September 2011

kivd (alpha-ketoisovalerate decarboxylase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 578
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding sequence, those RE site should not exist in the coding region.There is one EcoR1 site in kivd. The RE site in the part has been modified by point mutation.



Source

It is cloned form Lactococcus lactis subsp. lactis KF147

References