Difference between revisions of "Part:BBa K590059:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Lux C, [https://parts.igem.org/Part:BBa_K590061 Lux D] and [https://parts.igem.org/Part:BBa_K590061 Lux E] were re-optimized to have cloning-friendly GC content. Primers were designed to synthesize the genes separately each in their own PCR reaction. Another PCR was used to amplify the respective pieces, and each piece was ligated into psb1C3. Once each gene was in psb1C3, it was re-amplified out and ligated into a vector containing ADC. This is done step wise until the full construct is obtained. | |
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===Source=== | ===Source=== | ||
Revision as of 23:51, 20 September 2011
LuxC; standalone, E. Coli codon optimized
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Lux C, Lux D and Lux E were re-optimized to have cloning-friendly GC content. Primers were designed to synthesize the genes separately each in their own PCR reaction. Another PCR was used to amplify the respective pieces, and each piece was ligated into psb1C3. Once each gene was in psb1C3, it was re-amplified out and ligated into a vector containing ADC. This is done step wise until the full construct is obtained.
Source
Gene-synthesis