Difference between revisions of "Part:BBa K590018:Experience"
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| − | This part was submitted because we want to show that we were able to use Gibson cloning method to make multiple gene insert. We also envisioned that this part can be combined with the other parts to make a circuit that contains all the essential genes. | + | This part was submitted because we want to show that we were able to use Gibson cloning method to make multiple gene insert. We performed Gibson Cloning to combine the 5 genes, which were extracted as 3 inserts of manageable size, in a [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590014 pGA3k3] backbone. We also envisioned that this part can be combined with the other parts to make a circuit that contains all the essential genes. |
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Latest revision as of 23:08, 20 September 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K590018
User Reviews
This part was submitted because we want to show that we were able to use Gibson cloning method to make multiple gene insert. We performed Gibson Cloning to combine the 5 genes, which were extracted as 3 inserts of manageable size, in a pGA3k3 backbone. We also envisioned that this part can be combined with the other parts to make a circuit that contains all the essential genes.
UNIQ93c6a5dd9f63a243-partinfo-00000000-QINU UNIQ93c6a5dd9f63a243-partinfo-00000001-QINU