Difference between revisions of "Part:BBa K568000"
| Line 2: | Line 2: | ||
<partinfo>BBa_K568000 short</partinfo> | <partinfo>BBa_K568000 short</partinfo> | ||
| + | Single red light sensor without additional genes. This part can pe clonend anywhere one wants to. | ||
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | Red light induces the autophosphorylation at the cytosolic site of cph8. This leads to phosphorylation of OmpR which subsequently binds to OmpC promotor and enables transcription of a following gene. | |
| + | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K568000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K568000 SequenceAndFeatures</partinfo> | ||
| − | |||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K568000 parameters</partinfo> | <partinfo>BBa_K568000 parameters</partinfo> | ||
| − | + | ||
| + | Induction with red light at 705 nm | ||
| + | |||
| + | Since sensor is constitutive switched on, red light signalling can be shut down with 650 nm. | ||
Revision as of 22:23, 20 September 2011
Red light sensor
Single red light sensor without additional genes. This part can pe clonend anywhere one wants to.
Usage and Biology
Red light induces the autophosphorylation at the cytosolic site of cph8. This leads to phosphorylation of OmpR which subsequently binds to OmpC promotor and enables transcription of a following gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 644
Illegal XhoI site found at 1786 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 971
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
| n/a | Red light sensor |
Induction with red light at 705 nm
Since sensor is constitutive switched on, red light signalling can be shut down with 650 nm.