Difference between revisions of "Part:BBa K568005"
 
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Cloning intermediate. Last step of assembly.
 
Cloning intermediate. Last step of assembly.
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This is an intermediate cloning construct. For the final part (<html><a href="https://parts.igem.org/Part:BBa_K568001">BBa_K568001</a></html>) T7ptag, a T7 polymerase with amber stop codon mutation, (<html><a href="https://parts.igem.org Part:BBa_K228000">BBa_K228000</a></html>) needs to be added.
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This is an intermediate cloning construct. For (<html><a href="https://parts.igem.org/Part:BBa_K568001">BBa_K568001</a></html>), the final part, T7ptag, a T7 polymerase with amber stop codon mutation, (<html><a href="https://parts.igem.org Part:BBa_K228000">BBa_K228000</a></html>) needs to be added.
  
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 22:06, 20 September 2011

Intermediate optogenetical AND-Gate without T7ptag

Cloning intermediate. Last step of assembly. This is an intermediate cloning construct. For (BBa_K568001), the final part, T7ptag, a T7 polymerase with amber stop codon mutation, (BBa_K228000) needs to be added.

Usage and Biology

see Part (BBa_K568001)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
    Illegal XhoI site found at 1786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 971
    Illegal AgeI site found at 3817
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3820