Difference between revisions of "Part:BBa K568005"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K568005 short</partinfo>
 
<partinfo>BBa_K568005 short</partinfo>
  
 
Cloning intermediate. Last step of assembly.
 
Cloning intermediate. Last step of assembly.
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This is an intermediate cloning construct. For the final part (<html><a href="https://parts.igem.org/Part:BBa_K568001">BBa_K568001</a></html>) T7ptag, a T7 polymerase with amber stop codon mutation, (<html><a href="https://parts.igem.org Part:BBa_K228000">BBa_K228000</a></html>) needs to be added.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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see Part (<html><a href="https://parts.igem.org/Part:BBa_K568001">BBa_K568001</a></html>)
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K568005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K568005 SequenceAndFeatures</partinfo>

Revision as of 22:04, 20 September 2011

Intermediate optogenetical AND-Gate without T7ptag

Cloning intermediate. Last step of assembly. This is an intermediate cloning construct. For the final part (BBa_K568001) T7ptag, a T7 polymerase with amber stop codon mutation, (BBa_K228000) needs to be added.

Usage and Biology

see Part (BBa_K568001)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
    Illegal XhoI site found at 1786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 971
    Illegal AgeI site found at 3817
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3820