Difference between revisions of "Part:BBa K568003"

 
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__NOTOC__
 
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<partinfo>BBa_K568003 short</partinfo>
 
<partinfo>BBa_K568003 short</partinfo>
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beta-galactosidase expression upon T7 polymerase binding
 
beta-galactosidase expression upon T7 polymerase binding
  
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===Usage and Biology===
 
===Usage and Biology===
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This part can be used as a reporter plasmid. It expresses beta-galactosidase if there is a T7 polymerase (or a mutant variant) nearby or transcribed through another part.
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The polymerase binds to the T7 promoter and transcription of the lacZ-Gene can occur, leading to beta-galactosidase expression.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K568003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K568003 SequenceAndFeatures</partinfo>

Revision as of 21:58, 20 September 2011

T7 promoter lacZ reporter part

beta-galactosidase expression upon T7 polymerase binding


Usage and Biology

This part can be used as a reporter plasmid. It expresses beta-galactosidase if there is a T7 polymerase (or a mutant variant) nearby or transcribed through another part. The polymerase binds to the T7 promoter and transcription of the lacZ-Gene can occur, leading to beta-galactosidase expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]