Difference between revisions of "Part:BBa K553001:Design"

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===References===
 
===References===
 +
Piper KR, Beck von Bodman S, Far- rand SK. 1993. ''Conjugation factor of Agrobacterium tumefaciens regulates Ti plasmid transfer by autoinduction''. Nature 362:448–50
 +
 +
Zhang L, Murphy PJ, Kerr A, Tate ME. 1993. ''Agrobacterium conjugation and gene regulation by N-acyl-L-homoserine lactones''. Nature 362:446–48

Revision as of 21:50, 20 September 2011

A. tumefaciens TraI - OC8 HLA synthase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 385
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Obtained via PCR from Agrobacterium tumefaciens gDNA

TraI fw

5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCTGATTCTGACCGTCTCGCC 3'

TraI rev

5' GTTTCTTCCTGCAGCGGCCGCTACTAGTATCACGCCGCACTCCTCAACG 3'


pcr protocol:

95° 5' | 10x (93° 30" | 56° 30" | 72° 40") | 23x ( (93° 30" | 65° 30" | 72° 40") | 72° 7' | 4° ∞

Source

http://www.ncbi.nlm.nih.gov/gene/6382103

References

Piper KR, Beck von Bodman S, Far- rand SK. 1993. Conjugation factor of Agrobacterium tumefaciens regulates Ti plasmid transfer by autoinduction. Nature 362:448–50

Zhang L, Murphy PJ, Kerr A, Tate ME. 1993. Agrobacterium conjugation and gene regulation by N-acyl-L-homoserine lactones. Nature 362:446–48