Difference between revisions of "Part:BBa K678053"

Revision as of 21:31, 20 September 2011

pJEJAM5

pJEJAM5 is a plasmid intended for transient transfection of mammalian cells. pJEJAM3 consists of the strong constitutive cytomegalovirus (CMV) promoter, cyan fluorescent protein (CFP), the BGK polyA, the hygR marker cassette, an ori for propagation in E.coli and, ampR resistance cassette (Figure 1).

DTU-Denmark-2 2011 Figure 1: Plasmid map of pJEJAM5, the figure is not drawn to scale.

DTU-Denmark-2 2011 Figure 2: .

DTU-Denmark-2 2011 Figure 3: .

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 2207
Illegal PstI site found at 1021
Illegal PstI site found at 2297
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2207
Illegal PstI site found at 1021
Illegal PstI site found at 2297
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2207
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 2207
Illegal PstI site found at 1021
Illegal PstI site found at 2297
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 2207
Illegal PstI site found at 1021
Illegal PstI site found at 2297
Illegal NgoMIV site found at 3062
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K678053 short</partinfo>
-To be announced
+pJEJAM5 is a plasmid intended for transient transfection of mammalian cells. pJEJAM3 consists of the strong constitutive cytomegalovirus (CMV) promoter, cyan fluorescent protein (CFP), the ''BGK'' polyA, the ''hygR'' marker cassette, an ori for propagation in ''E.coli'' and, ampR resistance cassette (Figure 1).
+<br>
+<br>
+[[Image:Devices_16.png|none|500px|thumb|<b>DTU-Denmark-2 2011</b> Figure 1: Plasmid map of pJEJAM5, the figure is not drawn to scale.]]
+<br>
+<br>
+[[Image:16_033.png|left|300px|thumb|<b>DTU-Denmark-2 2011</b> Figure 2: .]]
+[[Image:16_blå.png|right|300px|thumb|<b>DTU-Denmark-2 2011</b> Figure 3: .]]
+<br clear=all>
 <!-- Add more about the biology of this part here