Difference between revisions of "Part:BBa K590017:Design"
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===Source=== | ===Source=== | ||
| − | Made from Gibson | + | Made from Gibson Cloning Method. See [http://2011.igem.org/Team:Washington/Magnetosomes/GibsonVectors Gibson Assembly Toolkit] |
===References=== | ===References=== | ||
Latest revision as of 21:07, 20 September 2011
mamHIEJKL in vector pGA3K3
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 5175
Illegal PstI site found at 8836 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 5175
Illegal PstI site found at 8836 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 8540
Illegal XhoI site found at 89
Illegal XhoI site found at 6375 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 5175
Illegal PstI site found at 8836 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 5175
Illegal PstI site found at 8836
Illegal NgoMIV site found at 238
Illegal NgoMIV site found at 1254
Illegal NgoMIV site found at 2589
Illegal NgoMIV site found at 3920
Illegal NgoMIV site found at 6754
Illegal NgoMIV site found at 8562
Illegal NgoMIV site found at 8929
Illegal AgeI site found at 5849
Illegal AgeI site found at 6264 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 651
Illegal BsaI site found at 1986
Illegal BsaI site found at 3317
Illegal BsaI.rc site found at 6947
Illegal SapI site found at 825
Illegal SapI site found at 2160
Illegal SapI site found at 3491
Illegal SapI.rc site found at 9208
Illegal SapI.rc site found at 9529
Design Notes
Partial sequence confirmed, microscopy shows cells chain formation with this plasmid
Source
Made from Gibson Cloning Method. See [http://2011.igem.org/Team:Washington/Magnetosomes/GibsonVectors Gibson Assembly Toolkit]