Difference between revisions of "Part:BBa K525222:Design"

(→‎Design Notes)
(→‎Source)
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* PCR on the Corynebacterium halotolerans DNA cloned into BioBrick Vectors
 
* PCR on the Corynebacterium halotolerans DNA cloned into BioBrick Vectors
 
** Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.  
 
** Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.  
−
*** fwd: GAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAAATGATCAAGAACCGCATCCGCAC
+
*** fwd: GAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAAATGCAGGAAACCACTGTAACCACC
−
*** rev: CTGCAGCGGCCGCTACTAGTATTAGCGGTTCAGGTTCAGGAAG
+
*** rev: CTGCAGCGGCCGCTACTAGTATTAGATGCCCAGGCCACCGACG
−
 
+
  
 
===References===
 
===References===

Revision as of 20:41, 20 September 2011

S-layer cspB from Corynebacterium halotolerans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1103
    Illegal XhoI site found at 559
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 226
    Illegal NgoMIV site found at 1315
    Illegal AgeI site found at 217
    Illegal AgeI site found at 458
    Illegal AgeI site found at 505
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 907
    Illegal BsaI.rc site found at 214
    Illegal BsaI.rc site found at 592
    Illegal BsaI.rc site found at 994


Design Notes

This is the core cspB S-layer sequence without TAT sequence and without lipid anchor.

Source

  • PCR on the Corynebacterium halotolerans DNA cloned into BioBrick Vectors
    • Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
      • fwd: GAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAAATGCAGGAAACCACTGTAACCACC
      • rev: CTGCAGCGGCCGCTACTAGTATTAGATGCCCAGGCCACCGACG

References