Difference between revisions of "Part:BBa K678049"
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pJEJAM1 is a plasmid intended for transient transfection of mammalian cells. pJEJAM1 consists of the strong constitutive cytomegalovirus (CMV) promoter, the green fluorescence protein (GFP), the ''BGK'' polyA and the ''hygR'' marker cassette used for selection, an ori for propagation in ''E.coli'' and and ampR resistance cassette (Figure below).
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pJEJAM1 is a plasmid intended for transient transfection of mammalian cells. pJEJAM1 consists of the strong constitutive cytomegalovirus (CMV) promoter, green fluorescence protein (GFP), the ''BGK'' polyA, the ''hygR'' marker cassette used for selection, an ori for propagation in ''E.coli'' and and ampR resistance cassette (Figure 1).
  
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[[Image:Device13.png|none|500px|thumb|<b>DTU-Denmark-2 2011</b> Figure 1: Plasmid map of pJEJAM15, the figure is not drawn to scale.]]
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consists of RFP with a target signal to the nucleus which can explain the spots compared to the results from device and the spores with a single nuclei contains a lot of RFP signal. We cannot conclude that the signal is accumulated in the nucleus since they are not dyed, though can it be concluded that the RFP signal is target a specific place and accumulated somewhere.
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[[Image:IGEM_001.png|left|300px|thumb|<b>DTU-Denmark-2 2011</b> Figure 2: .]]
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[[Image:IGEM3.png|right|300px|thumb|<b>DTU-Denmark-2 2011</b> Figure 3: .]]
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Revision as of 20:38, 20 September 2011

pJEJAM1

pJEJAM1 is a plasmid intended for transient transfection of mammalian cells. pJEJAM1 consists of the strong constitutive cytomegalovirus (CMV) promoter, green fluorescence protein (GFP), the BGK polyA, the hygR marker cassette used for selection, an ori for propagation in E.coli and and ampR resistance cassette (Figure 1).



DTU-Denmark-2 2011 Figure 1: Plasmid map of pJEJAM15, the figure is not drawn to scale.



consists of RFP with a target signal to the nucleus which can explain the spots compared to the results from device and the spores with a single nuclei contains a lot of RFP signal. We cannot conclude that the signal is accumulated in the nucleus since they are not dyed, though can it be concluded that the RFP signal is target a specific place and accumulated somewhere.

DTU-Denmark-2 2011 Figure 2: .
DTU-Denmark-2 2011 Figure 3: .



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2216
    Illegal PstI site found at 2306
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2216
    Illegal PstI site found at 2306
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2216
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2216
    Illegal PstI site found at 2306
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2216
    Illegal PstI site found at 2306
    Illegal NgoMIV site found at 3071
  • 1000
    COMPATIBLE WITH RFC[1000]