Difference between revisions of "Part:BBa K525222:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This is the | + | This is the core cspB S-layer sequence without TAT sequence and without lipid anchor. |
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===Source=== | ===Source=== | ||
Revision as of 20:38, 20 September 2011
S-layer cspB from Corynebacterium halotolerans
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1103
Illegal XhoI site found at 559 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 226
Illegal NgoMIV site found at 1315
Illegal AgeI site found at 217
Illegal AgeI site found at 458
Illegal AgeI site found at 505 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 907
Illegal BsaI.rc site found at 214
Illegal BsaI.rc site found at 592
Illegal BsaI.rc site found at 994
Design Notes
This is the core cspB S-layer sequence without TAT sequence and without lipid anchor.
Source
- PCR on the Corynebacterium halotolerans DNA cloned into BioBrick Vectors
- Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
- fwd: GAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAAATGATCAAGAACCGCATCCGCAC
- rev: CTGCAGCGGCCGCTACTAGTATTAGCGGTTCAGGTTCAGGAAG
- Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.