Difference between revisions of "Part:BBa K525123:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K525123 short</partinfo> | <partinfo>BBa_K525123 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | + | This is cspB S-layer sequence with the lipid anchor but without the TAT sequence. | |
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===Source=== | ===Source=== | ||
− | + | * PCR on the Corynebacterium glutamicum (ATCC 14067) DNA cloned into BioBrick Vectors | |
+ | ** Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer. | ||
+ | *** fwd: GAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAAATGCAGGAAACCACTGTAACCACC | ||
+ | *** rev: CTGCAGCGGCCGCTACTAGTATTAGAACTTAACGATACCGGAGAGG | ||
+ | * [http://www.ncbi.nlm.nih.gov/nuccore/AY842007.1 GenBank: AY842007.1] | ||
===References=== | ===References=== |
Revision as of 20:06, 20 September 2011
S-layer cspB from Corynebacterium glutamicum with lipid anchor and PT7 and RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1334
Illegal XhoI site found at 161
Illegal XhoI site found at 779 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1313
Illegal SapI site found at 560
Illegal SapI site found at 772
Illegal SapI site found at 1320
Design Notes
This is cspB S-layer sequence with the lipid anchor but without the TAT sequence.
Source
- PCR on the Corynebacterium glutamicum (ATCC 14067) DNA cloned into BioBrick Vectors
- Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
- fwd: GAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAAATGCAGGAAACCACTGTAACCACC
- rev: CTGCAGCGGCCGCTACTAGTATTAGAACTTAACGATACCGGAGAGG
- Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
- [http://www.ncbi.nlm.nih.gov/nuccore/AY842007.1 GenBank: AY842007.1]