Difference between revisions of "Part:BBa K525123:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K525123 short</partinfo>
 
<partinfo>BBa_K525123 short</partinfo>
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===Design Notes===
 
===Design Notes===
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PCR
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This is cspB S-layer sequence with the lipid anchor but without the TAT sequence.
  
  
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===Source===
 
===Source===
  
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Brevibacterium flavum, PCR
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* PCR on the Corynebacterium glutamicum (ATCC 14067) DNA cloned into BioBrick Vectors
 +
** Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
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*** fwd: GAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAAATGCAGGAAACCACTGTAACCACC
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*** rev: CTGCAGCGGCCGCTACTAGTATTAGAACTTAACGATACCGGAGAGG
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* [http://www.ncbi.nlm.nih.gov/nuccore/AY842007.1 GenBank: AY842007.1]
  
 
===References===
 
===References===

Revision as of 20:06, 20 September 2011

S-layer cspB from Corynebacterium glutamicum with lipid anchor and PT7 and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1334
    Illegal XhoI site found at 161
    Illegal XhoI site found at 779
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1313
    Illegal SapI site found at 560
    Illegal SapI site found at 772
    Illegal SapI site found at 1320


Design Notes

This is cspB S-layer sequence with the lipid anchor but without the TAT sequence.


Source

  • PCR on the Corynebacterium glutamicum (ATCC 14067) DNA cloned into BioBrick Vectors
    • Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
      • fwd: GAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAAATGCAGGAAACCACTGTAACCACC
      • rev: CTGCAGCGGCCGCTACTAGTATTAGAACTTAACGATACCGGAGAGG
  • [http://www.ncbi.nlm.nih.gov/nuccore/AY842007.1 GenBank: AY842007.1]

References