Difference between revisions of "Part:BBa K525121:Design"
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* PCR on the Corynebacterium glutamicum (ATCC 14067) DNA cloned into BioBrick Vectors | * PCR on the Corynebacterium glutamicum (ATCC 14067) DNA cloned into BioBrick Vectors | ||
| − | ** Primers with BioBrick | + | ** Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer. |
| − | *** | + | *** fwd: GAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAAATGTTTAACAACCGTATCCGCAC |
| − | *** | + | *** rev: CTGCAGCGGCCGCTACTAGTATTAGAACTTAACGATACCGGAGAGG |
* [http://www.ncbi.nlm.nih.gov/nuccore/AY842007.1 GenBank: AY842007.1] | * [http://www.ncbi.nlm.nih.gov/nuccore/AY842007.1 GenBank: AY842007.1] | ||
===References=== | ===References=== | ||
Revision as of 19:53, 20 September 2011
S-layer cspB from Corynebacterium glutamicum with TAT-Sequence and lipid anchor, PT7 and RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1421
Illegal XhoI site found at 248
Illegal XhoI site found at 866 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1400
Illegal SapI site found at 647
Illegal SapI site found at 859
Illegal SapI site found at 1407
Design Notes
PCR
Source
- PCR on the Corynebacterium glutamicum (ATCC 14067) DNA cloned into BioBrick Vectors
- Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
- fwd: GAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAAATGTTTAACAACCGTATCCGCAC
- rev: CTGCAGCGGCCGCTACTAGTATTAGAACTTAACGATACCGGAGAGG
- Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
- [http://www.ncbi.nlm.nih.gov/nuccore/AY842007.1 GenBank: AY842007.1]