Difference between revisions of "Part:BBa K592011"

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<partinfo>BBa_K592011 short</partinfo>
 
<partinfo>BBa_K592011 short</partinfo>
  
This chromoprotein from the ''Cnidopus japonicus'' sea anemone, cjBlue, naturally exhibits dark green color when expressed. Compared to amilCP(<partinfo>BBa_K592009</partinfo>) and amilGFP(<partinfo>BBa_K592010</partinfo>), the color development is slower. On agar plates and in liquid culture, the color is readily visible to naked eye after 24-48 hours of incubation. The DNA was codon optimized for expression in ''E coli'' and synthesized by the Korean company Bioneer Corporation.  
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This chromoprotein from the ''Cnidopus japonicus'' sea anemone, cjBlue, naturally exhibits dark green color when expressed. Compared to amilCP (<partinfo>BBa_K592009</partinfo>) and amilGFP (<partinfo>BBa_K592010</partinfo>), the color development is slower. On agar plates and in liquid culture, the color is readily visible to naked eye after 24-48 hours of incubation. The sequence is codon optimized for expression in ''E coli''.
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 19:50, 20 September 2011

cjBlue, green chromoprotein

This chromoprotein from the Cnidopus japonicus sea anemone, cjBlue, naturally exhibits dark green color when expressed. Compared to amilCP (BBa_K592009) and amilGFP (BBa_K592010), the color development is slower. On agar plates and in liquid culture, the color is readily visible to naked eye after 24-48 hours of incubation. The sequence is codon optimized for expression in E coli.

Usage and Biology

This part is useful as a reporter.

CjBlue plate small.jpg

Escherichia coli constitutively expressing cjBlue (BBa_K592011).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]