Difference between revisions of "Part:BBa K525121:Design"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K525121 short</partinfo>
 
<partinfo>BBa_K525121 short</partinfo>
Line 13: Line 12:
 
===Source===
 
===Source===
  
−
Brevibacterum flavum
+
* PCR on the Corynebacterium glutamicum (ATCC 14067) DNA cloned into BioBrick Vectors
 +
 
  
 
===References===
 
===References===

Revision as of 19:35, 20 September 2011

S-layer cspB from Corynebacterium glutamicum with TAT-Sequence and lipid anchor, PT7 and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1421
    Illegal XhoI site found at 248
    Illegal XhoI site found at 866
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1400
    Illegal SapI site found at 647
    Illegal SapI site found at 859
    Illegal SapI site found at 1407


Design Notes

PCR


Source

  • PCR on the Corynebacterium glutamicum (ATCC 14067) DNA cloned into BioBrick Vectors


References