Difference between revisions of "Part:BBa K678063"

 
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K678063 short</partinfo>
 
<partinfo>BBa_K678063 short</partinfo>
  
To be announced
+
Device consists of RFP with a target signal to the nucleus which can explain the spots compared to the results from device and the spores with a single nuclei contains a lot of RFP signal. We cannot conclude that the signal is accumulated in the nucleus since they are not dyed, though can it be concluded that the RFP signal is target a specific place and accumulated somewhere.
 +
<br>
 +
<br>
 +
<p align="center">
 +
<img src="https://static.igem.org/mediawiki/2011/a/ac/Device_25.png" height="100px" align="center"> </img>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<table cellpadding="10px" align="center">
 +
 
 +
<tr>
 +
<td><img src="https://static.igem.org/mediawiki/2011/c/c6/25_dic_t_1_png.png" height="200px" align="center"> </img></td>
 +
<td><img src="https://static.igem.org/mediawiki/2011/9/91/25_RFP_3-t%C3%B8r_colour_-2.jpg" height="200px" align="center"> </img></td>
 +
</tr>
 +
<tr>
 +
<td><i>Aspergillus nidulans </i> with device <br><a href="https://parts.igem.org/Part:BBa_K678063">BBa_K678063</a> - DIC light.</td>
 +
<td><i>Aspergillus nidulans </i> with device <br><a href="https://parts.igem.org/Part:BBa_K678063">BBa_K678063</a> - RFP light.</td>
 +
</tr>
 +
</table>
 +
 
 +
<table cellpadding="10px" align="center">
 +
 
 +
<tr>
 +
<td><img src="https://static.igem.org/mediawiki/2011/2/2f/25.14_front_rettet.jpg" height="200px" align="center"> </img></td>
 +
<td><img src="https://static.igem.org/mediawiki/2011/c/c8/25.14_back_rettet.jpg" height="200px" align="center"> </img></td>
 +
</tr>
 +
<tr>
 +
<td><i>Aspergillus nidulans </i> with device <br> <a href="https://parts.igem.org/Part:BBa_K678063">BBa_K678063</a> - Front</td>
 +
<td><i>Aspergillus nidulans </i> with device <br> <a href="https://parts.igem.org/Part:BBa_K678063">BBa_K678063</a> - Back </td>
 +
</tr>
 +
</table>
 +
</p>
 +
 
 +
<br>
 +
<br>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:32, 20 September 2011

pJEJAM15

Device consists of RFP with a target signal to the nucleus which can explain the spots compared to the results from device and the spores with a single nuclei contains a lot of RFP signal. We cannot conclude that the signal is accumulated in the nucleus since they are not dyed, though can it be concluded that the RFP signal is target a specific place and accumulated somewhere.

<img src="Device_25.png" height="100px" align="center"> </img>

<img src="25_dic_t_1_png.png" height="200px" align="center"> </img> <img src="25_RFP_3-t%C3%B8r_colour_-2.jpg" height="200px" align="center"> </img>
Aspergillus nidulans with device
<a href="https://parts.igem.org/Part:BBa_K678063">BBa_K678063</a> - DIC light.
Aspergillus nidulans with device
<a href="https://parts.igem.org/Part:BBa_K678063">BBa_K678063</a> - RFP light.
<img src="25.14_front_rettet.jpg" height="200px" align="center"> </img> <img src="25.14_back_rettet.jpg" height="200px" align="center"> </img>
Aspergillus nidulans with device
<a href="https://parts.igem.org/Part:BBa_K678063">BBa_K678063</a> - Front
Aspergillus nidulans with device
<a href="https://parts.igem.org/Part:BBa_K678063">BBa_K678063</a> - Back



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3747
    Illegal XbaI site found at 5303
    Illegal XbaI site found at 8047
    Illegal PstI site found at 140
    Illegal PstI site found at 2657
    Illegal PstI site found at 4588
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 910
    Illegal PstI site found at 140
    Illegal PstI site found at 2657
    Illegal PstI site found at 4588
    Illegal NotI site found at 5260
    Illegal NotI site found at 8086
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 144
    Illegal BamHI site found at 3028
    Illegal BamHI site found at 4326
    Illegal XhoI site found at 700
    Illegal XhoI site found at 1082
    Illegal XhoI site found at 4186
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3747
    Illegal XbaI site found at 5303
    Illegal XbaI site found at 8047
    Illegal PstI site found at 140
    Illegal PstI site found at 2657
    Illegal PstI site found at 4588
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3747
    Illegal XbaI site found at 5303
    Illegal XbaI site found at 8047
    Illegal PstI site found at 140
    Illegal PstI site found at 2657
    Illegal PstI site found at 4588
    Illegal NgoMIV site found at 3664
    Illegal AgeI site found at 1289
    Illegal AgeI site found at 1437
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4379
    Illegal BsaI.rc site found at 1116
    Illegal BsaI.rc site found at 3378
    Illegal SapI site found at 322