Difference between revisions of "Part:BBa K627000:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K627000 short</partinfo> | <partinfo>BBa_K627000 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | This biobrick was built by PCR using the following PCR primers | + | This biobrick was built by PCR using the following PCR primers<br> |
| − | Forward primer: TAAATGAATTCGCGGCCGCTTCTAGATGCCTCAATATACTACTAAAC | + | Forward primer: TAAATGAATTCGCGGCCGCTTCTAGATGCCTCAATATACTACTAAAC<br> |
| − | Reverse primer: ATTTCTGCAGCGGCCGCTACTAGTATCAGCAAACCCTACTTAATTTC | + | Reverse primer: ATTTCTGCAGCGGCCGCTACTAGTATCAGCAAACCCTACTTAATTTC<br> |
To insert mdnED in the vector pSb1C3, the resulting PCR product and the vector were digested with the restriction enzymes, EcoRI and SpeI. | To insert mdnED in the vector pSb1C3, the resulting PCR product and the vector were digested with the restriction enzymes, EcoRI and SpeI. | ||
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===References=== | ===References=== | ||
| + | Ziemert, N., Ishida, K., Liaimer, A., Hertweck, C. & Dittmann, E. (2008). Ribosomal synthesis of tricyclic depsipeptides in bloom-forming cyanobacteria. Angewandte Chemie (International ed. in English) 47, 7756-9<br> | ||
| + | <br> | ||
| + | Ziemert, N., Ishida, K., Weiz, A., Hertweck, C. & Dittmann, E. (2010). Exploiting the natural diversity of microviridin gene clusters for discovery of novel tricyclic depsipeptides. Applied and environmental microbiology 76, 3568-74 | ||
Latest revision as of 08:52, 20 September 2011
ABC transporter and N-acetyltransferase from mdn-cluster
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 281
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 777
Illegal SapI.rc site found at 1098
Design Notes
This biobrick was built by PCR using the following PCR primers
Forward primer: TAAATGAATTCGCGGCCGCTTCTAGATGCCTCAATATACTACTAAAC
Reverse primer: ATTTCTGCAGCGGCCGCTACTAGTATCAGCAAACCCTACTTAATTTC
To insert mdnED in the vector pSb1C3, the resulting PCR product and the vector were digested with the restriction enzymes, EcoRI and SpeI.
Source
The BioBrick mdnDE as a part of the microviridin gene (mdn) cluster was isolated from Microcystis aeruginosa strain NIES-843.
References
Ziemert, N., Ishida, K., Liaimer, A., Hertweck, C. & Dittmann, E. (2008). Ribosomal synthesis of tricyclic depsipeptides in bloom-forming cyanobacteria. Angewandte Chemie (International ed. in English) 47, 7756-9
Ziemert, N., Ishida, K., Weiz, A., Hertweck, C. & Dittmann, E. (2010). Exploiting the natural diversity of microviridin gene clusters for discovery of novel tricyclic depsipeptides. Applied and environmental microbiology 76, 3568-74