Difference between revisions of "Part:BBa K510021"

 
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<partinfo>BBa_K510021 short</partinfo>
 
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The mini-Tn7-Gm-Lux artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a pBad/Lux operon (BBa_K325909) in its BioBrick cloning site (BCS). This plasmid can be used for visualization purposes or to brand a strain because of its insertion in the genome of the working organism and split the antibiotic resistance cassette with the temporary expression of Flp recombinase.  
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The mini-Tn7-Gm-Lux artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm ([https://parts.igem.org/Part:BBa_K510012 BBa_K510012]) by inserting a pBad/Lux operon ([https://parts.igem.org/Part:BBa_K325909 BBa_K325909]) in its BioBrick cloning site (BCS). This plasmid can be used for visualization purposes or to brand a strain because of its insertion in the genome of the working organism and split the antibiotic resistance cassette with the temporary expression of Flp recombinase.  
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[[Image:Mini-Tn7-Gm-K325909.png|700px|center]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 22:04, 19 September 2011

pUC18R6KT-miniTn7BB-Gm-Lux


The mini-Tn7-Gm-Lux artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a pBad/Lux operon (BBa_K325909) in its BioBrick cloning site (BCS). This plasmid can be used for visualization purposes or to brand a strain because of its insertion in the genome of the working organism and split the antibiotic resistance cassette with the temporary expression of Flp recombinase.

Mini-Tn7-Gm-K325909.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4528
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4528
    Illegal NheI site found at 5762
    Illegal NheI site found at 8728
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4534
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4528
    Illegal BglII site found at 3212
    Illegal BglII site found at 3483
    Illegal BglII site found at 3769
    Illegal BglII site found at 7726
    Illegal BamHI site found at 5701
    Illegal XhoI site found at 8556
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4528
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4528
    Illegal XbaI site found at 4543
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 5536
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 10115
    Illegal BsaI.rc site found at 1854
    Illegal BsaI.rc site found at 7124
    Illegal SapI site found at 518
    Illegal SapI site found at 5518
    Illegal SapI.rc site found at 10440