Difference between revisions of "Part:BBa K510017"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K510017 short</partinfo> | <partinfo>BBa_K510017 short</partinfo> | ||
− | The mini-Tn7-Gm-tetR artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a tetR/ptetR cassette (BBa_I732083) in its BioBrick cloning site (BCS). This plasmid can be used as inducible expression system in single copy by integrating the device in the genome of the working organism. | + | |
+ | The mini-Tn7-Gm-tetR artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm ([https://parts.igem.org/Part:BBa_K510012 BBa_K510012]) by inserting a tetR/ptetR cassette ([https://parts.igem.org/Part:BBa_I732083 BBa_I732083]) in its BioBrick cloning site (BCS). This plasmid can be used as inducible expression system in single copy by integrating the device in the genome of the working organism. | ||
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+ | [[Image:Mini-Tn7-Gm-I732083.png|700px|center]] | ||
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Revision as of 21:32, 19 September 2011
pUC18R6KT-miniTn7BB-Gm-tetR
The mini-Tn7-Gm-tetR artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a tetR/ptetR cassette (BBa_I732083) in its BioBrick cloning site (BCS). This plasmid can be used as inducible expression system in single copy by integrating the device in the genome of the working organism.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 4528
Illegal suffix found in sequence at 1 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4528
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4534 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4528
Illegal BglII site found at 3212
Illegal BglII site found at 3483
Illegal BglII site found at 3769 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 4528
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 4528
Illegal XbaI site found at 4543
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1854
Illegal SapI site found at 518