Difference between revisions of "Part:BBa K510016"

 
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<partinfo>BBa_K510016 short</partinfo>
 
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The mini-Tn7-Gm-pBad/araC artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a pBad/araC promoter(BBa_I0500) in its BioBrick cloning site (BCS). This plasmid can be used as inducible expression vector in single copy by integrating the device in the genome of the working organism.  
 
The mini-Tn7-Gm-pBad/araC artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a pBad/araC promoter(BBa_I0500) in its BioBrick cloning site (BCS). This plasmid can be used as inducible expression vector in single copy by integrating the device in the genome of the working organism.  
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[[Image:Mini-Tn7-Gm-I0500.png|700px|center]]
  
 
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Revision as of 21:21, 19 September 2011

pUC18R6KT-miniTn7BB-Gm-pBad/araC


The mini-Tn7-Gm-pBad/araC artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a pBad/araC promoter(BBa_I0500) in its BioBrick cloning site (BCS). This plasmid can be used as inducible expression vector in single copy by integrating the device in the genome of the working organism.

Mini-Tn7-Gm-I0500.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4528
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4528
    Illegal NheI site found at 5762
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4534
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4528
    Illegal BglII site found at 3212
    Illegal BglII site found at 3483
    Illegal BglII site found at 3769
    Illegal BamHI site found at 5701
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4528
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4528
    Illegal XbaI site found at 4543
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 5536
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1854
    Illegal SapI site found at 518
    Illegal SapI site found at 5518