Difference between revisions of "Part:BBa K538203:Design"

Revision as of 19:10, 19 September 2011

pLacI + RBS + CspA

LacI:CspA K538203

This part is one of team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011]'s cold resistance BioBricks, also known as a CryoBrick. When transformed into E. coli, it should bestow a certain degree of cold resistance. It was placed under control of the Lac operon's promoter (pLacI: BBa_R0010) so that it may be expressed constitutively in LacI-null strains, or inducibly in LacI-capable strains. This promoter was taken from 2011's Spring Distribution Kit, as was the incorporated RBS. The coding region of this Brick is a new submission to the registry, also submitted separately as BBa_K538003.

For more information about the subparts' purpose and design, refer to their main page or design page, respectively.

BBa_R0010
- Main Page
- Design Page
BBa_B0034
- Main Page
- Design Page
BBa_K538001
- Main Page
- Design Page

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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−	This part is one of team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011]'s cold resistance BioBricks, also known as a CryoBrick. When transformed into ''E. coli'', it should bestow a certain degree of cold resistance. It was placed under control of the Lac operon's promoter (''pLacI'': [[Part:BBa_R0010\|BBa_R0010]]) so that it may be expressed constitutively in ''LacI''-null strains, or inducibly in ''LacI''-capable strains. This promoter was taken from 2011's Spring Distribution Kit, as was the incorporated RBS. The coding region of this Brick is a new submission to the registry, also submitted separately as [[Part:~~BBa_K538001~~\|BBa_K538003]].	+	This part is one of team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011]'s cold resistance BioBricks, also known as a CryoBrick. When transformed into ''E. coli'', it should bestow a certain degree of cold resistance. It was placed under control of the Lac operon's promoter (''pLacI'': [[Part:BBa_R0010\|BBa_R0010]]) so that it may be expressed constitutively in ''LacI''-null strains, or inducibly in ''LacI''-capable strains. This promoter was taken from 2011's Spring Distribution Kit, as was the incorporated RBS. The coding region of this Brick is a new submission to the registry, also submitted separately as [[Part:BBa_K538003\|BBa_K538003]].

	For more information about the subparts' purpose and design, refer to their main page or design page, respectively.		For more information about the subparts' purpose and design, refer to their main page or design page, respectively.