Difference between revisions of "Part:BBa K538203:Design"

 
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<partinfo>BBa_K538203 short</partinfo>
 
<partinfo>BBa_K538203 short</partinfo>
  
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<partinfo>BBa_K538203 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K538203 Icon</partinfo>
  
  
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===Design Notes===
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This part is one of team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011]'s cold resistance BioBricks, also known as a CryoBrick. When transformed into ''E. coli'', it should bestow a certain degree of cold resistance. It was placed under control of the Lac operon's promoter (''pLacI'': [[Part:BBa_R0010|BBa_R0010]]) so that it may be expressed constitutively in ''LacI''-null strains, or inducibly in ''LacI''-capable strains. This promoter was taken from 2011's Spring Distribution Kit, as was the incorporated RBS. The coding region of this Brick is a new submission to the registry, also submitted separately as [[Part:BBa_K538001|BBa_K538003]].
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For more information about the subparts' purpose and design, refer to their main page or design page, respectively.
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* '''BBa_R0010'''
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** [[Part:BBa_R0010|Main Page]]
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** [[Part:BBa_R0010:Design|Design Page]]
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* '''BBa_B0034'''
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** [[Part:BBa_B0034|Main Page]]
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** [[Part:BBa_B0034:Design|Design Page]]
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* '''BBa_K538001'''
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** [[Part:BBa_K538003|Main Page]]
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** [[Part:BBa_K538003:Design|Design Page]]
  
  
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===Source===
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<partinfo>BBa_K538203 SequenceAndFeatures</partinfo>
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===References===
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Revision as of 19:10, 19 September 2011

pLacI + RBS + CspA

LacI:CspA
K538203


This part is one of team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011]'s cold resistance BioBricks, also known as a CryoBrick. When transformed into E. coli, it should bestow a certain degree of cold resistance. It was placed under control of the Lac operon's promoter (pLacI: BBa_R0010) so that it may be expressed constitutively in LacI-null strains, or inducibly in LacI-capable strains. This promoter was taken from 2011's Spring Distribution Kit, as was the incorporated RBS. The coding region of this Brick is a new submission to the registry, also submitted separately as BBa_K538003.

For more information about the subparts' purpose and design, refer to their main page or design page, respectively.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]